An innovative chemical strategy for PCR-free genetic detection of pathogens by an integrated electrochemical biosensor.
نویسندگان
چکیده
An innovative chemical strategy integrated in a miniaturized electrochemical device was developed for sensitive detection of a pathogen genome (HBV virus) without any amplification step. The results show a limit of detection comparable to the standard qRT-PCR method (20 copies per reaction), paving the way to future development of genetic PoC devices addressing automatized and low-cost molecular diagnostics.
منابع مشابه
Facile Synthesis and Electrochemical Performance of Graphene-Modified Cu2O Nanocomposite for Use in Enzyme-Free Glucose Biosensor
Graphene-modified Cu2O nanocomposite was synthesized under facile microwave irradiation of an aqueous solution and has been investigated as an enzyme-free glucose biosensor. Morphology and crystal structure of the graphene-modified Cu2O nanocomposite were investigated by using electron microscopy and X-Ray Diffraction (XRD) analyses. Also, the electrochemical performan...
متن کاملLabel-free electrochemical biosensors for food and drug application
In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...
متن کاملLabel-free electrochemical biosensors for food and drug application
In food sector, there is a huge demand for rapid, reliable, user & eco-friendly biosensors to analyse the quality and safety of food products. Biosensor based methodology depends upon the recognition of a specific antigens or receptors by corresponding antibodies, aptamers or high-affinity ligands. The first scientifically commercialised sensors were the electrochemical sensors used for the ana...
متن کاملDesigning a Label Free Aptasensor for Detection of Methamphetamine
A label-free electrochemical nucleic acid aptasensor for the detection of methamphetamine (MA) by the immobilization of thiolated self-assembled DNA sequences on a gold nanoparticles-chitosan modified electrode is constructed. When MA was complexed specifically to the aptamer, the configuration of the nucleic acid aptamer switched to a locked structure and the interface of the biosensor changed...
متن کاملAn Aptamer-based Biosensor for Troponin I Detection in Diagnosis of Myocardial Infarction
Background: Acute myocardial infarction (MI) accounts for one third of deaths. Cardiac troponin I (TnI) is a reliable biomarker of cardiac muscle tissue injury and is employed in the early diagnosis of MI.Objectives: In this study, a molecular method is introduced to early diagnosis of MI by rapid detection of TnI.Materials and Methods: The detection method was based on electrochemical aptasens...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Analyst
دوره 142 12 شماره
صفحات -
تاریخ انتشار 2017